Multiple high-frequency transpositions of Tn5 in Serratia marcescens 274 from a thermosensitive replication mutant of plasmid R388

G R Estepa; R M Harshey

doi:10.1016/0378-1119(89)90196-0

Multiple high-frequency transpositions of Tn5 in Serratia marcescens 274 from a thermosensitive replication mutant of plasmid R388

Gene. 1989 Sep 30;81(2):355-9. doi: 10.1016/0378-1119(89)90196-0.

Authors

G R Estepa¹, R M Harshey

Affiliation

¹ Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.

PMID: 2553541
DOI: 10.1016/0378-1119(89)90196-0

Abstract

We have used the broad-host-range conjugative suicide plasmid vector described by Sasakawa and Yoshikawa [Gene 56 (1987) 283-288] for transposon mutagenesis of Serratia marcescens 274. We report multiple transposition events of Tn5 from this vector. In addition, the unusual pattern of Tn5 transposition might provide an insight into its regulation.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Blotting, Southern
Conjugation, Genetic
DNA Transposable Elements*
Hot Temperature
Mutation*
Plasmids / genetics*
Serratia marcescens / genetics*

Substances

DNA Transposable Elements