BODIPY-based fluorescent thermometer as a lysosome-targetable probe: how the oligo(ethylene glycols) compete photoinduced electron transfer

Chemistry. 2015 Feb 16;21(8):3219-23. doi: 10.1002/chem.201405853. Epub 2014 Dec 29.

Abstract

A novel BODIPY-based fluorescent thermometer, which shows a lysosome-targeting property, was successfully prepared. Due to the electron-donating ability of the oligo(ethylene glycols), the photoinduced electron-transfer pathway from morpholine to BODIPY dye is blocked. The fluorescence of the thermometer quenched by intramolecular rotation at room temperature was progressively enhanced during heating due to the increased microviscosity around the fluorophore.

Keywords: BODIPY; analytical methods; electron transfer; fluorescent probes; lysosome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Boron Compounds / chemistry*
  • Electron Transport
  • Ethylene Glycols / chemistry*
  • Fluorescent Dyes / chemistry*
  • Lysosomes / chemistry*
  • Lysosomes / metabolism
  • Morpholines / chemistry*
  • Thermometers

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Ethylene Glycols
  • Fluorescent Dyes
  • Morpholines