Strain-specific interaction of a GII.10 Norovirus with HBGAs

Virology. 2015 Feb:476:386-394. doi: 10.1016/j.virol.2014.12.039. Epub 2015 Jan 12.

Abstract

Noroviruses (NoVs), an important cause of gastroenteritis in humans, recognize human histo-blood group antigens (HBGAs) as receptors. The crystal structures of the protruding (P) domain of a GII.10 NoV (Vietnam 026) in complex with various HBGA oligosaccharides were elucidated. However, the HBGA binding profile of this virus remains unknown. In this study, we determined the saliva and oligosaccharide binding profiles of this virus and the roles of amino acids that are involved in HBGA binding. Our data showed that Vietnam 026 bound to all ABO secretor and non-secretor saliva with clear signals detected by monoclonal antibodies against H3, H1, Le(y), Le(a) and sialyl Le(a). Mutagenesis study confirmed the binding site determined by the crystallography study, in which single mutations wiped out the binding function. We also identified amino acids surrounding the central binding pocket that may participate in the binding by affecting the HBGA binding specificity of the GII.10 NoV.

Keywords: Acute gastroenteritis; Human histo-blood group antigens; Mutagenesis; Noroviruses; Oligosaccharide; Saliva.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blood Group Antigens / genetics
  • Blood Group Antigens / metabolism*
  • Caliciviridae Infections / metabolism*
  • Caliciviridae Infections / virology
  • Gastroenteritis / metabolism*
  • Gastroenteritis / virology
  • Humans
  • Models, Molecular
  • Molecular Sequence Data
  • Norovirus / genetics
  • Norovirus / metabolism*
  • Protein Binding
  • Protein Structure, Tertiary
  • Receptors, Virus / genetics
  • Receptors, Virus / metabolism*
  • Species Specificity
  • Viral Nonstructural Proteins / genetics
  • Viral Nonstructural Proteins / metabolism*

Substances

  • Blood Group Antigens
  • Receptors, Virus
  • Viral Nonstructural Proteins