Evaluation of a PCR targeting fimbrial subunit gene (fimA) for rapid and reliable detection of Enteroaggregative Escherichia coli recovered from human and animal diarrhoeal cases

Deepak B Rawool; Jess Vergis; Deepthi Vijay; Pankaj Dhaka; Mamta Negi; Manesh Kumar; Amruta Nair; Krupali V Poharkar; Nitin V Kurkure; Ashok Kumar; S V S Malik; S B Barbuddhe

doi:10.1016/j.mimet.2015.01.008

Evaluation of a PCR targeting fimbrial subunit gene (fimA) for rapid and reliable detection of Enteroaggregative Escherichia coli recovered from human and animal diarrhoeal cases

J Microbiol Methods. 2015 Mar:110:45-8. doi: 10.1016/j.mimet.2015.01.008. Epub 2015 Jan 15.

Authors

Affiliations

¹ Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India. Electronic address: [email protected].
² Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
³ Department of Veterinary Pathology, Nagpur Veterinary College, MAFSU, Nagpur 440 006, India.
⁴ National Institute of Biotic Stress Management, Raipur, Chhattisgarh 492 012, India.

PMID: 25598135
DOI: 10.1016/j.mimet.2015.01.008

Abstract

The present study describes the utility of a chromosomal associated fimbrial subunit gene (fimA) for screening 'typical' as well as 'atypical' Enteroaggregative Escherichia coli (EAEC) by PCR and its possible role as a promising molecular marker for rapid detection of 'typical' and 'atypical' EAEC pathotype.

Keywords: Diarrhoea; EAEC; HEp-2; PCR; fimA.

Publication types

Evaluation Study

MeSH terms

Animals
Diarrhea / microbiology*
Escherichia coli / genetics
Escherichia coli / isolation & purification*
Escherichia coli / ultrastructure
Escherichia coli Infections / microbiology
Escherichia coli Proteins / genetics*
Fimbriae Proteins / genetics
Fimbriae, Bacterial / genetics*
Humans
Polymerase Chain Reaction* / methods
Virulence / genetics

Substances

Escherichia coli Proteins
fimbrillin
Fimbriae Proteins