Abstract
In this study we identify a volatile compound produced by Pseudomonas aeruginosa, which can specifically activate the LuxR quorum-sensing response regulator of Vibrio fischeri. Comparative gas-chromatography analysis between P. aeruginosa wild type and a ΔlasR mutant strain implied that the active volatile is 2-aminoacetophenone. The use of synthetic 2-aminoacetophenone and in silico docking analyses verified the activity of the molecule and provided putative interacting residues within the binding site.
MeSH terms
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Acetophenones / metabolism*
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Aliivibrio fischeri / metabolism
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Biosensing Techniques
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Pseudomonas aeruginosa / metabolism*
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Quorum Sensing*
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Trans-Activators / genetics
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Trans-Activators / metabolism*
Substances
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Acetophenones
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Bacterial Proteins
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Repressor Proteins
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Trans-Activators
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LuxR autoinducer binding proteins
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2-aminoacetophenone