Abstract
This report focuses on two groups of cationic cancerostatics, anthracycline antibiotics and 1,4-benzoquinone-guanylhydrazone-thiosemicarbazone (ambazone), lining up biophysical and biochemical effects on the level of membranes and membrane constituents. The interaction of both drugs with multilamellar liposomes consisting of phosphatidylcholine used as a simple model membrane system could be ensured by means of steady state and nanosecond time-resolved fluorometric investigations. The biochemical effect on membranes is underlined by the inhibition of the neuraminidase activity of the Sendai virus, modification of the CAMP phosphodiesterase activity of leukemia L 1210 cells of mice and reduction of the lymphocyte blast transformation.
MeSH terms
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3',5'-Cyclic-AMP Phosphodiesterases / antagonists & inhibitors
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3',5'-Cyclic-AMP Phosphodiesterases / metabolism
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Adenylyl Cyclases / metabolism
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Aminoglycosides*
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Animals
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Anti-Bacterial Agents / metabolism
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Anti-Bacterial Agents / pharmacology
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Anti-Bacterial Agents / therapeutic use
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Antineoplastic Agents / metabolism
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Antineoplastic Agents / pharmacology*
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Antineoplastic Agents / therapeutic use
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Cell Division / drug effects
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Cell Membrane / drug effects
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Cell Transformation, Neoplastic / drug effects
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DNA / drug effects
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DNA / metabolism
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Daunorubicin / metabolism
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Daunorubicin / pharmacology
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Daunorubicin / therapeutic use
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Doxorubicin / metabolism
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Doxorubicin / pharmacology
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Doxorubicin / therapeutic use
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Fluorescence
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Humans
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Leukemia L1210 / drug therapy
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Leukemia L1210 / metabolism
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Leukemia L1210 / pathology
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Lymphocyte Activation / drug effects
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Lysosomes / drug effects
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Lysosomes / metabolism
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Macrophages / drug effects
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Macrophages / metabolism
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Mice
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Mitoguazone / analogs & derivatives
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Mitoguazone / metabolism
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Mitoguazone / pharmacology
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Mitoguazone / therapeutic use
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Neuraminidase / metabolism
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Parainfluenza Virus 1, Human / drug effects
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Parainfluenza Virus 1, Human / enzymology
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Spectrophotometry
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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Antineoplastic Agents
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violamycins
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Doxorubicin
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DNA
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1,4-benzoquinone guanylhydrazone thiosemicarbazone
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3',5'-Cyclic-AMP Phosphodiesterases
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Neuraminidase
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Adenylyl Cyclases
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Mitoguazone
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Daunorubicin