Induction of CAD gene amplification by restriction endonucleases in V79,B7 Chinese hamster cells

Mutat Res. 1989 Jan-Feb;225(1-2):61-4. doi: 10.1016/0165-7992(89)90034-1.

Abstract

The restriction endonucleases PvuII, BamHI and EcoRI were tested for their ability to induce gene amplification in V79,B7 Chinese hamster cells. The results indicate that treatment with these enzymes efficiently increases the frequency of clones resistant to N-phosphonacetyl-L-aspartate, indicating induction of CAD gene amplification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases / genetics*
  • Animals
  • Aspartate Carbamoyltransferase / genetics*
  • Aspartic Acid / analogs & derivatives*
  • Aspartic Acid / pharmacology
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / genetics*
  • Cell Line
  • Cricetinae
  • Cricetulus
  • DNA Restriction Enzymes / metabolism*
  • Dihydroorotase / genetics*
  • Gene Amplification*
  • Genes*
  • Multienzyme Complexes / genetics*
  • Mutagenicity Tests
  • Neoplasm Proteins / genetics
  • Organophosphorus Compounds / pharmacology*
  • Phosphonoacetic Acid / analogs & derivatives
  • Phosphonoacetic Acid / pharmacology*

Substances

  • CAD trifunctional enzyme
  • Multienzyme Complexes
  • Neoplasm Proteins
  • Organophosphorus Compounds
  • Aspartic Acid
  • sparfosic acid
  • Aspartate Carbamoyltransferase
  • DNA Restriction Enzymes
  • Amidohydrolases
  • Dihydroorotase
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
  • Phosphonoacetic Acid