Functional redundancy of protein kinase D1 and protein kinase D2 in neuronal polarity

Erda Avriyanti; Nur Atik; Masataka Kunii; Naomi Furumoto; Tomohiko Iwano; Shin-Ichiro Yoshimura; Reiko Harada; Akihiro Harada

doi:10.1016/j.neures.2015.01.007

Functional redundancy of protein kinase D1 and protein kinase D2 in neuronal polarity

Neurosci Res. 2015 Jun:95:12-20. doi: 10.1016/j.neures.2015.01.007. Epub 2015 Jan 29.

Authors

Erda Avriyanti¹, Nur Atik², Masataka Kunii¹, Naomi Furumoto³, Tomohiko Iwano¹, Shin-Ichiro Yoshimura¹, Reiko Harada⁴, Akihiro Harada⁵

Affiliations

¹ Department of Cell Biology, Graduate School of Medicine, Osaka University, Suita, 565-0871, Japan.
² Department of Cell Biology, Graduate School of Medicine, Osaka University, Suita, 565-0871, Japan; Department of Anatomy and Cell Biology, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia.
³ Laboratory for Molecular Traffic, Department of Cellular and Molecular Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, 371-8512, Japan.
⁴ Department of Cell Biology, Graduate School of Medicine, Osaka University, Suita, 565-0871, Japan; Department of Judo Therapy, Takarazuka University of Medical and Health Care, Takarazuka, 666-0162, Japan.
⁵ Department of Cell Biology, Graduate School of Medicine, Osaka University, Suita, 565-0871, Japan; Laboratory for Molecular Traffic, Department of Cellular and Molecular Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, 371-8512, Japan. Electronic address: [email protected].

PMID: 25639845
DOI: 10.1016/j.neures.2015.01.007

Abstract

Mammalian protein kinase D (PKD) isoforms have been proposed to regulate diverse biological processes, including the establishment and maintenance of neuronal polarity. To investigate the function of PKD in neuronal polarization in vivo, we generated PKD knockout (KO) mice. Here, we show that the brain, particularly the hippocampus, of both PKD1 KO and PKD2 KO mice was similar to that of control animals. Neurite length in cultured PKD1 KO and PKD2 KO hippocampal neurons was similar to that of wild-type neurons. However, hippocampal neurons deficient in both PKD1 and PKD2 genes showed a reduction in axonal elongation and an increase in the percentage of neurons with multiple axons relative to control neurons. These results reveal that whereas PKD1 and PKD2 are essential for neuronal polarity, there exists a functional redundancy between the two proteins.

Keywords: Functional redundancy; Knockout mouse; Neuronal polarity; Protein kinase D.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Axons / enzymology
Cell Polarity*
Cells, Cultured
Hippocampus / cytology
Hippocampus / enzymology*
Mice
Mice, Knockout
Neurons / cytology
Neurons / enzymology*
Protein Kinase C / genetics
Protein Kinase C / metabolism*
Protein Kinase D2
Protein Kinases / genetics
Protein Kinases / metabolism*

Substances

Protein Kinase D2
Protein Kinases
protein kinase D
Protein Kinase C