Complete genome sequence of yacon necrotic mottle virus, a novel putative member of the genus Badnavirus

Arch Virol. 2015 Apr;160(4):1139-42. doi: 10.1007/s00705-015-2341-2. Epub 2015 Feb 4.

Abstract

The complete genome sequence of a previously undescribed virus isolated from a yacon plant exhibiting necrotic mottle, chlorosis, stunting, and leaf malformation symptoms in Gyeongju, Korea, was determined. The genome of this virus consists of one circular double-stranded DNA of 7661 bp in size. The genome contained four open reading frames (ORFs 1 to 4) on the plus strand that potentially encode proteins of 26, 32, 234, and 25 kDa. Protein BLAST analysis showed that ORF3, which is the largest ORF, has 45 % amino acid sequence identity (with 89 % coverage) to the ORF3 of fig badnavirus 1 (FBV-1), a recently identified badnavirus. Phylogenetic analysis provided further evidence that the virus identified in this study is probably a member of a new species in the genus Badnavirus. The name yacon necrotic mottle virus (YNMoV) is proposed for this new virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Asteraceae / virology*
  • Badnavirus / chemistry
  • Badnavirus / classification
  • Badnavirus / genetics*
  • Badnavirus / isolation & purification
  • Base Sequence
  • Genome, Viral*
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • Plant Diseases / virology
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Viral Proteins / chemistry
  • Viral Proteins / genetics

Substances

  • Viral Proteins

Associated data

  • GENBANK/KM229702