Femtosecond laser bone ablation with a high repetition rate fiber laser source

Luke J Mortensen; Clemens Alt; Raphaël Turcotte; Marissa Masek; Tzu-Ming Liu; Daniel C Côté; Chris Xu; Giuseppe Intini; Charles P Lin

doi:10.1364/BOE.6.000032

Femtosecond laser bone ablation with a high repetition rate fiber laser source

Biomed Opt Express. 2014 Dec 5;6(1):32-42. doi: 10.1364/BOE.6.000032. eCollection 2015 Jan 1.

Authors

Luke J Mortensen¹, Clemens Alt², Raphaël Turcotte³, Marissa Masek², Tzu-Ming Liu⁴, Daniel C Côté⁵, Chris Xu⁶, Giuseppe Intini⁷, Charles P Lin⁸

Affiliations

¹ Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts, USA ; Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
² Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
³ Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA ; Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA.
⁴ Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA ; Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
⁵ Centre de Recherche Université Laval Robert-Giffard, Université Laval, Québec, QC G1J2G3, Canada.
⁶ School of Applied and Engineering Physics, Cornell University, Ithaca, New York, USA.
⁷ Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts, USA ; Harvard Stem Cell Institute, Cambridge, Massachusetts, USA ; [email protected] ; Co-corresponding authors.
⁸ Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA ; Harvard Stem Cell Institute, Cambridge, Massachusetts, USA ; Co-corresponding authors.

Abstract

Femtosecond laser pulses can be used to perform very precise cutting of material, including biological samples from subcellular organelles to large areas of bone, through plasma-mediated ablation. The use of a kilohertz regenerative amplifier is usually needed to obtain the pulse energy required for ablation. This work investigates a 5 megahertz compact fiber laser for near-video rate imaging and ablation in bone. After optimization of ablation efficiency and reduction in autofluorescence, the system is demonstrated for the in vivo study of bone regeneration. Image-guided creation of a bone defect and longitudinal evaluation of cellular injury response in the defect provides insight into the bone regeneration process.

Keywords: (060.4370) Nonlinear optics, fibers; (170.1020) Ablation of tissue; (170.2520) Fluorescence microscopy; (180.4315) Nonlinear microscopy.

Abstract

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