Phenotypic and molecular characterization of multidrug-resistant Salmonella enterica serovar Hadar in Greece, from 2007 to 2010

T Papadopoulos; E Petridou; A Zdragas; S Nair; T Peters; E de Pinna; G Mandilara; M Passiotou; A Vatopoulos

doi:10.1016/j.cmi.2014.09.016

Phenotypic and molecular characterization of multidrug-resistant Salmonella enterica serovar Hadar in Greece, from 2007 to 2010

Clin Microbiol Infect. 2015 Feb;21(2):149.e1-4. doi: 10.1016/j.cmi.2014.09.016. Epub 2014 Oct 13.

Authors

T Papadopoulos¹, E Petridou², A Zdragas³, S Nair⁴, T Peters⁴, E de Pinna⁴, G Mandilara⁵, M Passiotou⁶, A Vatopoulos⁵

Affiliations

¹ Laboratory of Microbiology, School of Veterinary Medicine, Aristotle University, Thessaloniki, Greece. Electronic address: [email protected].
² Laboratory of Microbiology, School of Veterinary Medicine, Aristotle University, Thessaloniki, Greece.
³ Veterinary Research Institute, National Agricultural Research Foundation, Thermi, Greece.
⁴ Salmonella Reference Service, Public Health England, London, UK.
⁵ National Reference Centre for Salmonella, National School of Public Health & Central Public Health Laboratory, Hellenic Centre of Disease Control and Prevention, Vari, Greece.
⁶ Veterinary Laboratory of Chalkis, Veterinary National Reference Laboratory for Salmonella, Chalkis, Greece.

PMID: 25658552
DOI: 10.1016/j.cmi.2014.09.016

Abstract

All 120 strains of Salmonella enterica serovar Hadar isolated during 2007-2010 in Greece were characterized by phenotypic and molecular methods. High rates of resistance to nalidixic acid (92%) and low levels of ciprofloxacin resistance (88%) were observed. Pulsenet-pulsed field gel electrophoresis profile SHADXB.0001 was predominant in Greece (58%) as in Europe but PT1, a rare phage type in Europe, was frequent in Greece (56%). The SHADXB.0001 and PT1 clone (38%) were found in humans, animals and food of animal origin with R-type ApSpTNxpCp being predominant (25%). The data indicate that this clone (possibly endemic) was circulating through the food chain in Greece during the study period.

Keywords: Antimicrobial susceptibility; Salmonella Hadar; clone; phage typing; pulsed field gel electrophoresis.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bacteriophage Typing
Drug Resistance, Bacterial
Electrophoresis, Gel, Pulsed-Field
Food Microbiology*
Genotype
Greece / epidemiology
Humans
Microbial Sensitivity Tests
Molecular Typing
Phenotype
Salmonella Infections / epidemiology*
Salmonella Infections / microbiology
Salmonella Infections, Animal / epidemiology*
Salmonella Infections, Animal / microbiology
Salmonella enterica / classification
Salmonella enterica / drug effects
Salmonella enterica / genetics
Salmonella enterica / isolation & purification*
Serogroup*

Substances

Anti-Bacterial Agents