Differentiation of germ cells in seminiferous tubules transplanted to testes of germ cell-deficient mice of W/Wv and Sl/Sld genotypes

J Cell Physiol. 1989 May;139(2):329-34. doi: 10.1002/jcp.1041390215.

Abstract

(WB X C57BL/6)F1-W/Wv (hereafter, WBB6F1-W/Wv) mice and (WC X C57BL/6)F1-Sl/Sld (hereafter, WCB6F1-Sl/Sld) mice are sterile due to the deficient spermatogenesis in the testes. The cause of deficient spermatogenesis in WBB6F1-W/Wv mice is considered to be a defect in germ cells themselves, whereas that in WCB6F1-Sl/Sld mice is considered to be a defect in tissue environment necessary for differentiation of germ cells. Seminiferous tubules isolated from cryptorchid testes of C57BL/6- +/+ mice were transplanted into the testes of WBB6F1-W/Wv and WCB6F1-Sl/Sld mice to clarify that the extratubular environment of these mice was intact or not. Type A spermatogonia in the transplanted tubules normally differentiated into spermatids, suggesting that the extratubular environment is intact in both WBB6F1-W/Wv and WCB6F1-Sl/Sld mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cryptorchidism / pathology*
  • Infertility, Male / genetics
  • Infertility, Male / pathology*
  • Leydig Cells / physiology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Seminiferous Tubules / cytology*
  • Seminiferous Tubules / physiology
  • Seminiferous Tubules / transplantation
  • Spermatogonia / cytology*
  • Spermatozoa / cytology*
  • Testis / cytology*
  • Testis / pathology
  • Testis / surgery