Mammalian cells were shown to fuse after direct electric pulsation of the plated cells in culture. The extent of fusion was controlled by the duration of the post-pulse incubation. Formation of polynucleated cells was slow, even at 37 degrees C. Pre-pulse incubation with colchicine increased the fusion yield slightly. Cytoskeletal organization during the post-pulse incubation was observed using immunofluorescence techniques. Microfilaments were unaffected, but microtubules disappeared during the first minutes following the pulse, and then reformed on subsequent incubation.