Annexin A2 is required for the early steps of cytokinesis

Christelle Benaud; Gaëlle Le Dez; Svetlana Mironov; Federico Galli; David Reboutier; Claude Prigent

doi:10.15252/embr.201440015

Annexin A2 is required for the early steps of cytokinesis

EMBO Rep. 2015 Apr;16(4):481-9. doi: 10.15252/embr.201440015. Epub 2015 Feb 23.

Authors

Christelle Benaud¹, Gaëlle Le Dez², Svetlana Mironov², Federico Galli², David Reboutier², Claude Prigent²

Affiliations

¹ Centre National de la Recherche Scientifique, UMR 6290 Equipe Labellisée Ligue 2014, Rennes, France Institut de Génétique et Développement de Rennes, Université de Rennes I, Rennes, France [email protected].
² Centre National de la Recherche Scientifique, UMR 6290 Equipe Labellisée Ligue 2014, Rennes, France Institut de Génétique et Développement de Rennes, Université de Rennes I, Rennes, France.

Abstract

Cytokinesis requires the formation of an actomyosin contractile ring between the two sets of sister chromatids. Annexin A2 is a calcium- and phospholipid-binding protein implicated in cortical actin remodeling. We report that annexin A2 accumulates at the equatorial cortex at the onset of cytokinesis and depletion of annexin A2 results in cytokinetic failure, due to a defective cleavage furrow assembly. In the absence of annexin A2, the small GTPase RhoA-which regulates cortical cytoskeletal rearrangement-fails to form a compact ring at the equatorial plane. Furthermore, annexin A2 is required for cortical localization of the RhoGEF Ect2 and to maintain the association between the equatorial cortex and the central spindle. Our results demonstrate that annexin A2 is necessary in the early phase of cytokinesis. We propose that annexin A2 participates in central spindle-equatorial plasma membrane communication.

Keywords: RhoA; annexin A2; cytokinesis; mitosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Annexin A2 / antagonists & inhibitors
Annexin A2 / genetics*
Annexin A2 / metabolism
Binding Sites
Cell Line, Tumor
Chromatids / metabolism
Chromatids / ultrastructure
Cytokinesis / genetics*
Gene Expression Regulation
Genes, Reporter
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
HeLa Cells
Humans
Luminescent Proteins / genetics
Luminescent Proteins / metabolism
Osteoblasts / metabolism*
Osteoblasts / ultrastructure
Point Mutation
Protein Binding
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins / metabolism
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Red Fluorescent Protein
S100 Proteins / genetics
S100 Proteins / metabolism
Signal Transduction
Spindle Apparatus / metabolism*
Spindle Apparatus / ultrastructure
rhoA GTP-Binding Protein / genetics
rhoA GTP-Binding Protein / metabolism

Substances

ANXA2 protein, human
Annexin A2
ECT2 protein, human
Luminescent Proteins
Proto-Oncogene Proteins
RNA, Small Interfering
Recombinant Proteins
S100 Proteins
S100 calcium binding protein A10
RHOA protein, human
Green Fluorescent Proteins
rhoA GTP-Binding Protein