Mice deficient for wild-type p53-induced phosphatase 1 display elevated anxiety- and depression-like behaviors

Neuroscience. 2015 May 7:293:12-22. doi: 10.1016/j.neuroscience.2015.02.037. Epub 2015 Feb 27.

Abstract

Mood disorders are a severe health burden but molecular mechanisms underlying mood dysfunction remain poorly understood. Here, we show that wild-type p53-induced phosphatase 1 (Wip1) negatively responds to the stress-induced negative mood-related behaviors. Specifically, we show that Wip1 protein but not its mRNA level was downregulated in the hippocampus but not in the neocortex after 4 weeks of chronic unpredictable mild stress (CUMS) in mice. Moreover, the CUMS-responsive WIP1 downregulation in the hippocampus was restored by chronic treatment of fluoxetine (i.p. 20 mg/kg) along with the CUMS procedure. In addition, Wip1 knockout mice displayed decreased exploratory behaviors as well as increased anxiety-like and depression-like behaviors in mice without impaired motor activities under the non-CUMS condition. Furthermore, the Wip1 deficiency-responsive anxiety-like but not depression-like behaviors were further elevated in mice under CUMS. Although limitations like male-alone sampling and multiply behavioral testing exist, the present study suggests a potential protective function of Wip1 in mood stabilization.

Keywords: CUMS; Wip1; anxiety-like behavior; depression-like behavior; exploratory behavior; fluoxetine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antidepressive Agents, Second-Generation / pharmacology
  • Anxiety / metabolism*
  • Anxiety / physiopathology
  • Depression / metabolism*
  • Depression / physiopathology
  • Exploratory Behavior / physiology
  • Fluoxetine / pharmacology
  • Hippocampus / drug effects
  • Hippocampus / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Phosphoprotein Phosphatases / genetics
  • Phosphoprotein Phosphatases / metabolism*
  • Protein Phosphatase 2C
  • RNA, Messenger / metabolism
  • Stress, Psychological / metabolism
  • Stress, Psychological / physiopathology

Substances

  • Antidepressive Agents, Second-Generation
  • RNA, Messenger
  • Fluoxetine
  • Phosphoprotein Phosphatases
  • Ppm1d protein, mouse
  • Protein Phosphatase 2C