Objective: To prepare and identify monoclonal antibody (mAb) against human telomere-associated repression and activation protein 1 (Rap1).
Methods: BALB/c mice were immunized with recombinant Rap1 protein. The spleen cells of the immunized mice were fused with Sp2/0 cells. The positive clones were confirmed and selected by indirect ELISA for titer determination and specificity identification.
Results: One hybridoma cell strain secreting specific mAb against Rap1 was obtained, namely 6F11. ELISA showed that the titer of the ascites was 1:10 000. Western blotting, immunoprecipitation and immunofluorescence experiments demonstrated that the mAb could specifically recognize and bind Rap1.
Conclusion: We have prepared mAb against human telomere-associated protein Rap1. The Rap1 mAb has a good binding ability and specificity.