Utility of recombinant fragment C for assessment of anti-tetanus antibodies in plasma

Diagn Microbiol Infect Dis. 2015 May;82(1):11-3. doi: 10.1016/j.diagmicrobio.2015.01.016. Epub 2015 Feb 7.

Abstract

Anti-tetanus antibodies in biological samples are typically detected using an enzyme-linked immunosorbent assay based on toxoided tetanus neurotoxin as antigen. We demonstrate that recombinantly produced fragment C of the toxin heavy chain is an effective alternative antigen for assessment of tetanus-immune status in plasma samples.

Keywords: ELISA; Fragment C; Immune response; Recombinant antigen; Tetanus; Vaccine surveillance.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Antitoxins / blood*
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Infant
  • Plasma / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology*
  • Recombinant Proteins / isolation & purification
  • Tetanus / immunology*
  • Tetanus / prevention & control*
  • Tetanus Toxin / genetics
  • Tetanus Toxin / immunology*

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Antitoxins
  • Recombinant Proteins
  • Tetanus Toxin