High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus

Theor Appl Genet. 2015 Jun;128(6):1039-47. doi: 10.1007/s00122-015-2488-y. Epub 2015 Mar 10.

Abstract

We characterise the distribution of crossover and non-crossover recombination in Brassica napus and Cicer arietinum using a low-coverage genotyping by sequencing pipeline SkimGBS. The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brassica napus / genetics*
  • Chromosome Mapping
  • Cicer / genetics*
  • Crossing Over, Genetic*
  • Gene Conversion*
  • Genome, Plant
  • Genotype
  • Genotyping Techniques*
  • High-Throughput Nucleotide Sequencing
  • Polymorphism, Single Nucleotide