Efficient Interaction between Arenavirus Nucleoprotein (NP) and RNA-Dependent RNA Polymerase (L) Is Mediated by the Virus Nucleocapsid (NP-RNA) Template

J Virol. 2015 May;89(10):5734-8. doi: 10.1128/JVI.00103-15. Epub 2015 Mar 11.

Abstract

In this study, we document that efficient interaction between arenavirus nucleoprotein (NP) and RNA-dependent RNA polymerase (L protein), the two trans-acting viral factors required for both virus RNA replication and gene transcription, requires the presence of virus-specific RNA sequences located within the untranslated 5' and 3' termini of the viral genome.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arenavirus / genetics
  • Arenavirus / metabolism*
  • Genome, Viral
  • HEK293 Cells
  • Humans
  • Lymphocytic choriomeningitis virus / genetics
  • Lymphocytic choriomeningitis virus / physiology
  • Nucleocapsid / genetics
  • Nucleocapsid / metabolism*
  • Nucleoproteins / genetics
  • Nucleoproteins / metabolism*
  • RNA, Untranslated / genetics
  • RNA, Untranslated / metabolism
  • RNA, Viral / genetics
  • RNA, Viral / metabolism
  • RNA-Dependent RNA Polymerase / genetics
  • RNA-Dependent RNA Polymerase / metabolism*
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*
  • Virus Replication

Substances

  • Nucleoproteins
  • RNA, Untranslated
  • RNA, Viral
  • Viral Proteins
  • RNA-Dependent RNA Polymerase