Abstract
Stem cell therapy requires a nontoxic and high-throughput method to achieve a pure cell population to prevent teratomas that can occur if even one cell in the implant has not been transformed. A promising method to detect and separate cells expressing a particular gene is RNA beacon technology. However, developing a successful, specific beacon to a particular transfected gene can take months to develop and in some cases is impossible. Here, we report on an off-the-shelf universal beacon that decreases the time and cost of applying beacon technology to select any living cell population transfected with an exogenous gene.
Keywords:
Fluorescence-activated cell sorting analysis; Gene expression; RNA beacons; Stem cell selection; Stem cell separation.
©AlphaMed Press.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Cell Tracking / methods
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Dogs
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Fluorescent Dyes / chemistry*
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Humans
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Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels / biosynthesis
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Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels / genetics
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Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels / isolation & purification*
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Mesenchymal Stem Cells / cytology*
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Molecular Probes / genetics
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Potassium Channels / biosynthesis
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Potassium Channels / genetics
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Potassium Channels / isolation & purification*
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RNA, Messenger / biosynthesis
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RNA, Messenger / isolation & purification*
Substances
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Fluorescent Dyes
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HCN2 protein, human
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Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
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Molecular Probes
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Potassium Channels
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RNA, Messenger