Abstract
Most annotated genomes show a large number of sense-antisense transcripts that can generate double-stranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Base Pairing
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Base Sequence
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Cloning, Molecular / methods*
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High-Throughput Nucleotide Sequencing
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Molecular Sequence Data
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Molecular Structure
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RNA, Double-Stranded / chemistry
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RNA, Double-Stranded / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Transcription, Genetic / physiology