LC-MS-MS Analysis of N,α-Diethylphenethylamine (N,α-ETH) and Its Positional Isomer N,β-Diethylphenethylamine (N,β-ETH) in Dietary Supplements

Mahmoud A ElSohly; Timothy P Murphy; Kareem M ElSohly; Waseem Gul

doi:10.1093/jat/bkv022

LC-MS-MS Analysis of N,α-Diethylphenethylamine (N,α-ETH) and Its Positional Isomer N,β-Diethylphenethylamine (N,β-ETH) in Dietary Supplements

J Anal Toxicol. 2015 Jun;39(5):387-406. doi: 10.1093/jat/bkv022. Epub 2015 Mar 19.

Authors

Mahmoud A ElSohly¹, Timothy P Murphy², Kareem M ElSohly³, Waseem Gul⁴

Affiliations

¹ ElSohly Laboratories, Inc. (ELI), 5 Industrial Park Drive, Oxford, MS 38655, USA Phytochemical Services, Incorporated (PSI), 5 Industrial Park Drive, Oxford, MS 38655, USA National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, University, MS 38677, USA [email protected].
² ElSohly Laboratories, Inc. (ELI), 5 Industrial Park Drive, Oxford, MS 38655, USA.
³ ElSohly Laboratories, Inc. (ELI), 5 Industrial Park Drive, Oxford, MS 38655, USA Phytochemical Services, Incorporated (PSI), 5 Industrial Park Drive, Oxford, MS 38655, USA.
⁴ ElSohly Laboratories, Inc. (ELI), 5 Industrial Park Drive, Oxford, MS 38655, USA Phytochemical Services, Incorporated (PSI), 5 Industrial Park Drive, Oxford, MS 38655, USA National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA.

PMID: 25792717
DOI: 10.1093/jat/bkv022

Abstract

In a previous publication, we reported on the analysis of several dietary supplement/exercise formulas and the quantitation of N,α-diethylphenethylamine (N,α-ETH, 3: ). In this article we report on the reanalysis of these products using LC-MS-MS and GC-MS methods capable of clearly separating the N,α-isomer ( 3: ) from its N,β-isomer (N,β-ETH, 4: ). The reanalysis, by both methods, showed that all samples previously reported as containing N,α-ETH ( 3: ) do contain only that isomer with no detectable concentrations of the N,β-ETH ( 4: ).

Publication types

Validation Study

MeSH terms

Calibration
Chromatography, Liquid* / standards
Dietary Supplements / analysis*
Gas Chromatography-Mass Spectrometry
Isomerism
Methamphetamine / analogs & derivatives*
Methamphetamine / analysis
Methamphetamine / chemistry
Molecular Structure
Reference Standards
Reproducibility of Results
Tandem Mass Spectrometry* / standards

Substances

N,alpha-diethylphenylethylamine
Methamphetamine