Sterols from Hericium erinaceum and their inhibition of TNF-α and NO production in lipopolysaccharide-induced RAW 264.7 cells

Phytochemistry. 2015 Jul:115:231-8. doi: 10.1016/j.phytochem.2015.02.021. Epub 2015 Mar 18.

Abstract

Erinarols G-J and 10 known ergostane-type sterols were isolated from a methanol extract of the dried fruiting bodies of Hericium erinaceum. Their chemical structures were elucidated using extensive spectroscopic analyses including 1D and 2D NMR experiments and HR-ESI-MS analysis, as well as through comparison with previously reported data. Anti-inflammatory effects of the isolated compounds were evaluated in terms of inhibition of tumor necrosis factor α (TNF-α) and nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cells. The results showed that erinarols H and J, as well as 2 of the ergostane-type sterols exhibited inhibitory activity against TNF-α secretion, with inhibition values ranging from 33.7% to 43.3% at 10 μM. Erinarols J and three ergostane-type sterols exhibited significant inhibitory effects against NO production, with inhibition values ranging from 38.4% to 71.5% at 10 μM.

Keywords: Anti-inflammation; Erinarol; Hericiaceae; Hericium erinaceum; NO; TNF-α.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basidiomycota / chemistry*
  • Electron Spin Resonance Spectroscopy
  • Ergosterol* / analogs & derivatives
  • Ergosterol* / chemistry
  • Ergosterol* / isolation & purification
  • Ergosterol* / pharmacology
  • Fruiting Bodies, Fungal / chemistry
  • Lipopolysaccharides / pharmacology
  • Mice
  • Nitric Oxide / biosynthesis
  • RAW 264.7 Cells
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors*

Substances

  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide
  • ergostane
  • Ergosterol