Abstract
A new phenotypic test, called the Carbapenem Inactivation Method (CIM), was developed to detect carbapenemase activity in Gram-negative rods within eight hours. This method showed high concordance with results obtained by PCR to detect genes coding for the carbapenemases KPC, NDM, OXA-48, VIM, IMP and OXA-23. It allows reliable detection of carbapenemase activity encoded by various genes in species of Enterobacteriaceae (e.g., Klebsiella pneumoniae, Escherichia coli and Enterobacter cloacae), but also in non-fermenters Pseudomonas aeruginosa and Acinetobacter baumannii. The CIM was shown to be a cost-effective and highly robust phenotypic screening method that can reliably detect carbapenemase activity.
MeSH terms
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Acinetobacter baumannii / drug effects
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Acinetobacter baumannii / enzymology
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Acinetobacter baumannii / genetics
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / antagonists & inhibitors
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Carbapenems / pharmacology
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DNA, Bacterial / analysis
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Enterobacteriaceae / drug effects
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Enterobacteriaceae / enzymology
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Enterobacteriaceae / genetics
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Gram-Negative Bacteria / drug effects
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Gram-Negative Bacteria / enzymology*
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Gram-Negative Bacteria / genetics
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High-Throughput Nucleotide Sequencing
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Phenotype
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Polymerase Chain Reaction
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Pseudomonas aeruginosa / drug effects
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Pseudomonas aeruginosa / enzymology
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Pseudomonas aeruginosa / genetics
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Sequence Analysis, DNA
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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DNA, Bacterial
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beta-Lactamases
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carbapenemase
Grants and funding
The authors have no support or funding to report.