It's a dirty job--A robust method for the purification and de novo genome assembly of Cryptosporidium from clinical material

Sofia Andersson; Per Sikora; Maria L Karlberg; Jadwiga Winiecka-Krusnell; Erik Alm; Jessica Beser; Romanico B G Arrighi

doi:10.1016/j.mimet.2015.03.018

It's a dirty job--A robust method for the purification and de novo genome assembly of Cryptosporidium from clinical material

J Microbiol Methods. 2015 Jun:113:10-2. doi: 10.1016/j.mimet.2015.03.018. Epub 2015 Mar 21.

Authors

Sofia Andersson¹, Per Sikora¹, Maria L Karlberg¹, Jadwiga Winiecka-Krusnell¹, Erik Alm¹, Jessica Beser¹, Romanico B G Arrighi²

Affiliations

¹ Public Health Agency of Sweden, Department of Microbiology, Solna, Sweden.
² Public Health Agency of Sweden, Department of Microbiology, Solna, Sweden. Electronic address: [email protected].

PMID: 25804506
DOI: 10.1016/j.mimet.2015.03.018

Abstract

We have developed a novel strategy for the purification of Cryptosporidium oocysts from clinical samples using IMS and PCR amplification of target DNA to facilitate uniform coverage genome sequencing and de novo assembly. Our procedure could also be used for other microbial pathogens from clinical specimens.

Keywords: Clinical parasitology; Cryptosporidium; Genome sequencing; Immunomagnetic separation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cryptosporidiosis / parasitology
Cryptosporidium / genetics*
Cryptosporidium / isolation & purification*
DNA, Protozoan / analysis
Feces / parasitology
Genome, Protozoan*
Humans
Immunomagnetic Separation*
Oocysts
Polymerase Chain Reaction*

Substances

DNA, Protozoan