Development of a clickable activity-based protein profiling (ABPP) probe for agmatine deiminases

Bioorg Med Chem. 2015 May 1;23(9):2159-67. doi: 10.1016/j.bmc.2015.03.013. Epub 2015 Mar 9.

Abstract

Agmatine deiminases (AgDs) catalyze the hydrolytic conversion of agmatine (decarboxylated arginine) to N-carbamoylputrescine with concomitant release of ammonia. These enzymes, which are encoded by some pathogenic bacterial species, confer a competitive survival advantage by virtue of energy production and acid tolerance through agmatine catabolism. Herein we report the development of a clickable activity-based protein profiling (ABPP) probe that targets the AgD encoded by Streptococcus mutans with high selectivity and sensitivity.

Keywords: ABPP probe; Activity-based protein profiling; Agmatine catabolism; Agmatine deiminase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocatalysis
  • Click Chemistry*
  • Drug Design
  • Hydrolases / analysis*
  • Hydrolases / metabolism*
  • Kinetics
  • Molecular Probes / analysis*
  • Molecular Probes / chemical synthesis
  • Molecular Probes / chemistry
  • Molecular Structure
  • Streptococcus mutans / enzymology*
  • Substrate Specificity

Substances

  • Molecular Probes
  • Hydrolases
  • agmatine deiminase