Us9-Independent Axonal Sorting and Transport of the Pseudorabies Virus Glycoprotein gM

J Virol. 2015 Jun;89(12):6511-4. doi: 10.1128/JVI.00625-15. Epub 2015 Apr 1.

Abstract

Axonal sorting and transport of fully assembled pseudorabies virus (PRV) virions is dependent on the viral protein Us9. Here we identify a Us9-independent mechanism for axonal localization of viral glycoprotein M (gM). We detected gM-mCherry assemblies transporting in the anterograde direction in axons. Furthermore, unlabeled gM, but not glycoprotein B, was detected by Western blotting in isolated axons during Us9-null PRV infection. These results suggest that gM differs from other viral proteins regarding axonal transport properties.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Axons / virology*
  • Herpesvirus 1, Suid / physiology*
  • Intracellular Signaling Peptides and Proteins
  • Lipoproteins / metabolism*
  • Phosphoproteins / metabolism*
  • Protein Transport*
  • Viral Envelope Proteins / metabolism*
  • Viral Proteins / metabolism*

Substances

  • Intracellular Signaling Peptides and Proteins
  • Lipoproteins
  • Phosphoproteins
  • US9 protein, Suid herpesvirus 1
  • Viral Envelope Proteins
  • Viral Proteins
  • pseudorabies virus glycoproteins