Hematopoietic Cell Transplantation Using Reduced-Intensity Conditioning Is Successful in Children with Hematologic Cytopenias of Genetic Origin

Biol Blood Marrow Transplant. 2015 Jul;21(7):1321-5. doi: 10.1016/j.bbmt.2015.03.019. Epub 2015 Mar 31.

Abstract

Genetically derived hematologic cytopenias are a rare heterogeneous group of disorders. Allogeneic hematopoietic cell transplantation (HCT) is curative but offset by organ toxicities from the preparative regimen, graft rejection, graft-versus-host disease (GVHD), or mortality. Because of these possibilities, consideration of HCT can be delayed, especially in the unrelated donor setting. We report a prospective multicenter trial of reduced-intensity conditioning (RIC) with alemtuzumab, fludarabine, and melphalan and HCT in 11 children with marrow failure of genetic origin (excluding Fanconi anemia) using the best available donor source (82% from unrelated donors). The median age at transplantation was 23 months (range, 2 months to 14 years). The median times to neutrophil (>500 × 10(6)/L) and platelet (>50 × 10(9)/L) engraftment were 13 (range, 12 to 24) and 30 (range, 7 to 55) days, respectively. The day +100 probability of grade II to IV acute GVHD and the 1-year probability of limited and extensive GVHD were 9% and 27%, respectively. The probability of 5-year overall and event-free survival was 82%; 9 patients were alive with normal blood counts at last follow-up and all were successfully off systemic immunosuppression. In patients with genetically derived severe hematologic cytopenias, allogeneic HCT with this RIC regimen was successful in achieving a cure. This experience supports consideration of HCT early in such patients even in the absence of suitable related donors.

Keywords: Congenital hematologic cytopenias; Hematopoietic cell transplantation; Reduced-intensity conditioning.

Publication types

  • Clinical Trial
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Adolescent
  • Alemtuzumab
  • Antibodies, Monoclonal, Humanized / therapeutic use
  • Child
  • Child, Preschool
  • Female
  • Graft vs Host Disease / etiology
  • Graft vs Host Disease / immunology
  • Graft vs Host Disease / mortality
  • Graft vs Host Disease / prevention & control
  • Hematologic Neoplasms / genetics
  • Hematologic Neoplasms / immunology
  • Hematologic Neoplasms / mortality
  • Hematologic Neoplasms / therapy*
  • Hematopoietic Stem Cell Transplantation / adverse effects*
  • Hematopoietic Stem Cell Transplantation / methods
  • Humans
  • Immunosuppressive Agents / therapeutic use
  • Infant
  • Male
  • Melphalan / therapeutic use
  • Myeloablative Agonists / therapeutic use*
  • Neutropenia / genetics
  • Neutropenia / immunology
  • Neutropenia / mortality
  • Neutropenia / therapy*
  • Prospective Studies
  • Risk
  • Siblings
  • Survival Analysis
  • Thrombocytopenia / genetics
  • Thrombocytopenia / immunology
  • Thrombocytopenia / mortality
  • Thrombocytopenia / therapy*
  • Transplantation Conditioning / methods*
  • Transplantation, Homologous
  • Treatment Outcome
  • Unrelated Donors
  • Vidarabine / analogs & derivatives
  • Vidarabine / therapeutic use

Substances

  • Antibodies, Monoclonal, Humanized
  • Immunosuppressive Agents
  • Myeloablative Agonists
  • Alemtuzumab
  • Vidarabine
  • fludarabine
  • Melphalan