MiR-195 suppresses non-small cell lung cancer by targeting CHEK1

Ben Liu; Jinli Qu; Fangxiu Xu; Yan Guo; Yu Wang; Herbert Yu; Biyun Qian

doi:10.18632/oncotarget.3255

MiR-195 suppresses non-small cell lung cancer by targeting CHEK1

Oncotarget. 2015 Apr 20;6(11):9445-56. doi: 10.18632/oncotarget.3255.

Authors

Ben Liu¹, Jinli Qu¹, Fangxiu Xu¹, Yan Guo¹, Yu Wang¹, Herbert Yu², Biyun Qian^{1

3}

Affiliations

¹ Department of Epidemiology and Biostatistics, Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Key Laboratory of Cancer Prevention and Therapy, Tianjin, National Clinical Research Center of Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.
² Cancer Epidemiology Program, University of Hawaii Cancer Center, Honolulu, HI 96813, USA.
³ Hongqiao International Institute of Medicine, Shanghai Tongren Hospital and Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Abstract

MiR-195 suppresses tumor growth and is associated with better survival outcomes in several malignancies including non-small cell lung cancer (NSCLC). Our previous study showed high miR-195 plasma levels associated with favorable overall survival of non-smoking women with lung adenocarcinoma. To further elucidate role of miR-195 in NSCLC, we conducted in vitro experiment as well as clinical studies in a cohort of 299 NSCLC samples. We demonstrated that miR-195 expression was lower in tumor tissues and was associated with poor survival outcome. Overexpression of miR-195 suppressed tumor cell growth, migration and invasion. We discovered that CHEK1 was a direct target of miR-195, which decreased CHEK1 expression in lung cancer cells. High expression of CHEK1 in lung tumors was associated with poor overall survival. Our results suggest that miR-195 suppresses NSCLC and predicts lung cancer prognosis.

Keywords: CHEK1; cell cycle; miR-195; non-small cell lung cancer; prognosis.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions / genetics
Adenocarcinoma / chemistry
Adenocarcinoma / genetics
Adenocarcinoma / mortality
Adult
Aged
Aged, 80 and over
Carcinoma, Non-Small-Cell Lung / chemistry
Carcinoma, Non-Small-Cell Lung / genetics*
Carcinoma, Non-Small-Cell Lung / mortality
Carcinoma, Squamous Cell / chemistry
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / mortality
Cell Cycle Checkpoints
Cell Division
Cell Line, Tumor
Cell Movement
Checkpoint Kinase 1
Cyclins / biosynthesis
Cyclins / genetics
Down-Regulation
Female
Follow-Up Studies
Gene Expression Regulation, Neoplastic
Humans
Kaplan-Meier Estimate
Lung Neoplasms / chemistry
Lung Neoplasms / genetics*
Lung Neoplasms / mortality
Male
MicroRNAs / genetics*
Middle Aged
Neoplasm Proteins / biosynthesis
Neoplasm Proteins / genetics*
Prognosis
Protein Kinases / biosynthesis
Protein Kinases / genetics*
RNA Interference
RNA, Small Interfering / genetics
Transfection

Substances

3' Untranslated Regions
Cyclins
MIRN195 microRNA, human
MicroRNAs
Neoplasm Proteins
RNA, Small Interfering
Protein Kinases
CHEK1 protein, human
Checkpoint Kinase 1