Matrix-assisted laser desorption/ionization time of flight mass-spectrometry (MALDI-TOF MS) based typing of extended-spectrum β-lactamase producing E. coli--a novel tool for real-time outbreak investigation

Adrian Egli; Sarah Tschudin-Sutter; Michael Oberle; Daniel Goldenberger; Reno Frei; Andreas F Widmer

doi:10.1371/journal.pone.0120624

Matrix-assisted laser desorption/ionization time of flight mass-spectrometry (MALDI-TOF MS) based typing of extended-spectrum β-lactamase producing E. coli--a novel tool for real-time outbreak investigation

PLoS One. 2015 Apr 10;10(4):e0120624. doi: 10.1371/journal.pone.0120624. eCollection 2015.

Authors

Adrian Egli¹, Sarah Tschudin-Sutter², Michael Oberle³, Daniel Goldenberger⁴, Reno Frei⁴, Andreas F Widmer²

Affiliations

¹ Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland; Infection Biology Lab, Department Biomedicine, University of Basel, Basel, Switzerland.
² Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.
³ Clinical Microbiology, Cantonal Hospital Aarau, Aarau, Switzerland.
⁴ Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.

Abstract

Epidemiologically linked clusters are confirmed by typing strains with molecular typing such as pulsed-field gel electrophoresis (PFGE). We compared six extended-spectrum β-lactamase producing E. coli of a PFGE-related cluster with Matrix-assisted laser desorption/ionization-time of flight mass-spectrometry based typing that confirmed relatedness faster and more cost-effective, but as reliable as PFGE.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Bacterial Typing Techniques
Cluster Analysis
Disease Outbreaks
Electrophoresis, Gel, Pulsed-Field
Escherichia coli / classification
Escherichia coli / enzymology*
Escherichia coli / isolation & purification
Escherichia coli Infections / diagnosis
Escherichia coli Infections / epidemiology
Escherichia coli Infections / microbiology
Humans
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
beta-Lactamases / analysis*
beta-Lactamases / metabolism

Substances

beta-Lactamases

Grants and funding

A.E. research was supported by a grant from the University of Basel “Nachwuchsförderung”. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.