Mangrovibacterium marinum sp. nov., isolated from a coastal sediment

Antonie Van Leeuwenhoek. 2015 Jun;107(6):1583-9. doi: 10.1007/s10482-015-0452-0. Epub 2015 Apr 12.

Abstract

A Gram-stain negative, facultatively anaerobic, non-motile and rod shaped bacterium, designated FA423(T), was isolated from a marine sediment obtained off the coast of Weihai, China. Strain FA423(T) was found to grow at 17-42 °C (optimum, 33 °C) and pH 5.5-8.0 (optimum, 7.0-7.5) in the presence of 0.5-6.0 % (w/v) NaCl (optimum, 1.0-2.0 %). Cells were found to be catalase positive and oxidase negative. Phylogenetic analysis of its 16S rRNA gene sequence revealed that strain FA423(T) is a member of the genus Mangrovibacterium and exhibits the highest sequence similarity (95.9 %) to the type strain of Mangrovibacterium diazotrophicum. The dominant cellular fatty acids were identified as summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B) and iso-C15:0. Menaquinone MK-7 was found to be the sole respiratory quinone. The major polar lipids were found to be an unidentified lipid and an unidentified phospholipid. The DNA G+C content of strain FA423(T) is 47.4 mol%. Based on the phylogenetic and phenotypic characteristics, strain FA423(T) is considered to represent a novel species of the genus Mangrovibacterium, for which the name Mangrovibacterium marinum sp. nov. is proposed. The type strain is FA423(T) (=DSM 28823(T) = KCTC 42253(T) = CICC 10903(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaerobiosis
  • Bacterial Typing Techniques
  • Bacteroidetes / classification*
  • Bacteroidetes / genetics
  • Bacteroidetes / isolation & purification*
  • Bacteroidetes / physiology
  • Base Composition
  • China
  • Cluster Analysis
  • Cytosol / chemistry
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Geologic Sediments / microbiology*
  • Hydrogen-Ion Concentration
  • Microscopy, Electron, Transmission
  • Molecular Sequence Data
  • Phospholipids / analysis
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sodium Chloride / metabolism
  • Temperature
  • Vitamin K 2 / analysis

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • Sodium Chloride

Associated data

  • GENBANK/KP214057