Molecular characterization of ISCR1-mediated blaPER-1 in a non-O1, non-O139 Vibrio cholerae strain from China

Jun Wu; Lianyan Xie; Fangfang Zhang; Yuxing Ni; Jingyong Sun

doi:10.1128/AAC.00166-15

Molecular characterization of ISCR1-mediated blaPER-1 in a non-O1, non-O139 Vibrio cholerae strain from China

Antimicrob Agents Chemother. 2015 Jul;59(7):4293-5. doi: 10.1128/AAC.00166-15. Epub 2015 Apr 13.

Authors

Jun Wu¹, Lianyan Xie², Fangfang Zhang², Yuxing Ni², Jingyong Sun³

Affiliations

¹ Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
² Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
³ Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China [email protected].

Abstract

We report the detection of PER-1 extended-spectrum β-lactamase (ESBL) in a clinical non-O1, non-O139 Vibrio cholerae strain from China. ISCR1-mediated bla(PER-1) was embedded in a complex In4 family class 1 integron belonging to the lineage of Tn1696 on a conjugative IncA/C plasmid. A free 8.98-kb circular molecule present with the ISCR1-bla(PER-1)-truncated 3'-conserved sequence (CS) structure was detected in this isolate. These findings may provide insight into the mobilization of bla(PER-1).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

China
Cholera / microbiology
Conserved Sequence
DNA Transposable Elements / genetics*
Humans
Integrons / genetics
Molecular Sequence Data
Mutagenesis, Insertional
Plasmids / genetics
Polymerase Chain Reaction
Vibrio cholerae / genetics*
Vibrio cholerae non-O1 / genetics*
beta-Lactamases / genetics*
beta-Lactamases / isolation & purification

Substances

DNA Transposable Elements
beta-lactamase PER-1
beta-Lactamases