Development of a viability standard curve for microencapsulated probiotic bacteria using confocal microscopy and image analysis software

Sarah Moore; Kasipathy Kailasapathy; Michael Phillips; Mark R Jones

doi:10.1016/j.mimet.2015.04.002

Development of a viability standard curve for microencapsulated probiotic bacteria using confocal microscopy and image analysis software

J Microbiol Methods. 2015 Jul:114:16-22. doi: 10.1016/j.mimet.2015.04.002. Epub 2015 Apr 14.

Authors

Sarah Moore¹, Kasipathy Kailasapathy², Michael Phillips³, Mark R Jones⁴

Affiliations

¹ Science Department, School of Health & Science, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia.
² Science Department, School of Health & Science, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia. Electronic address: [email protected].
³ Science Department, School of Health & Science, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia. Electronic address: [email protected].
⁴ Science Department, School of Health & Science, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia. Electronic address: [email protected].

PMID: 25887694
DOI: 10.1016/j.mimet.2015.04.002

Abstract

Microencapsulation is proposed to protect probiotic strains from food processing procedures and to maintain probiotic viability. Little research has described the in situ viability of microencapsulated probiotics. This study successfully developed a real-time viability standard curve for microencapsulated bacteria using confocal microscopy, fluorescent dyes and image analysis software.

Keywords: Confocal microscopy; Image analysis software; Microencapsulation; Probiotic bacteria; Viability standard curve.

MeSH terms

Image Processing, Computer-Assisted / methods*
Microbial Viability*
Microscopy, Confocal / methods*
Probiotics*
Software
Technology, Pharmaceutical / methods*