Abstract
A highly sensitive test system, based on the method of immuno-PCR, was developed for the detection of two staphylococcal toxins: enterotoxin A (SEA) and toxic shock syndrome toxin (TSST). A key element of the developed systems was obtaining supramolecular complexes of bisbiotinylated oligodeoxynucleotides and streptavidin, which were used as DNA tags. Specificity studies showed no cross-reactivity when determining SEA and TSST. The sensitivity of detection of these toxins in the culture supernatants S. aureus was not less than 10 pg/mL.
MeSH terms
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Bacterial Toxins / chemistry
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Bacterial Toxins / genetics
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Bacterial Toxins / isolation & purification*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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Enterotoxins / chemistry
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Enterotoxins / genetics
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Enterotoxins / isolation & purification*
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Enzyme-Linked Immunosorbent Assay
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Humans
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Polymerase Chain Reaction / methods*
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Shock, Septic / diagnosis
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Shock, Septic / microbiology
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Staphylococcal Infections / diagnosis*
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Staphylococcal Infections / microbiology
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Staphylococcus aureus / chemistry
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Staphylococcus aureus / genetics
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Staphylococcus aureus / pathogenicity
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Superantigens / chemistry
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Superantigens / genetics
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Superantigens / isolation & purification*
Substances
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Bacterial Toxins
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DNA, Bacterial
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Enterotoxins
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Superantigens
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enterotoxin F, Staphylococcal
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enterotoxin A, Staphylococcal