Multiple cyp51A-based mechanisms identified in azole-resistant isolates of Aspergillus fumigatus from China

Musang Liu; Rong Zeng; Lili Zhang; Dongmei Li; Guixia Lv; Yongnian Shen; Hailin Zheng; Qiangqiang Zhang; Jingjun Zhao; Nan Zheng; Weida Liu

doi:10.1128/AAC.00003-15

Multiple cyp51A-based mechanisms identified in azole-resistant isolates of Aspergillus fumigatus from China

Antimicrob Agents Chemother. 2015 Jul;59(7):4321-5. doi: 10.1128/AAC.00003-15. Epub 2015 Apr 20.

Authors

Musang Liu¹, Rong Zeng¹, Lili Zhang¹, Dongmei Li², Guixia Lv¹, Yongnian Shen¹, Hailin Zheng¹, Qiangqiang Zhang³, Jingjun Zhao⁴, Nan Zheng⁵, Weida Liu⁶

Affiliations

¹ Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China.
² Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China Georgetown University Medical Center, Department of Microbiology and Immunology, Washington, DC, USA.
³ Dermatology Department, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.
⁴ Dermatology Department, Tongji Hospital, Tongji University, Shanghai, People's Republic of China.
⁵ Medical School, Nanjing University, Nanjing, Jiangsu, People's Republic of China.
⁶ Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China [email protected].

Abstract

Seventy-two A. fumigatus clinical isolates from China were investigated for azole resistance based on mutations of cyp51A. We identified four azole-resistant strains, among which we found three strains highly resistant to itraconazole, two of which exhibit the TR34/L98H/S297T/F495I mutation, while one carries only the TR34/L98H mutation. To our knowledge, the latter has not been found previously in China. The fourth multiazole-resistant isolate (with only moderate itraconazole resistance) carries a new G432A mutation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antifungal Agents / pharmacology*
Aspergillosis / microbiology*
Aspergillus fumigatus / drug effects*
Aspergillus fumigatus / genetics
Azoles / pharmacology*
China
Cytochrome P-450 Enzyme System / genetics*
Cytochrome P-450 Enzyme System / metabolism*
Drug Resistance, Fungal / drug effects*
Fungal Proteins / genetics*
Fungal Proteins / metabolism*
Genotype
Humans
Itraconazole / pharmacology
Microbial Sensitivity Tests
Molecular Sequence Data
Mutation / genetics

Substances

Antifungal Agents
Azoles
Fungal Proteins
Itraconazole
Cytochrome P-450 Enzyme System
cytochrome P-450 CYP51A, Aspergillus