Sox4 participates in the modulation of Schwann cell myelination

Eur J Neurosci. 2015 Jul;42(2):1788-96. doi: 10.1111/ejn.12929. Epub 2015 May 19.

Abstract

In order to identify new regulators of Schwann cell myelination potentially playing a role in peripheral nervous system (PNS) pathologies, we analysed gene expression profiling data from three mouse models of demyelinating neuropathies and from the developing PNS. This analysis revealed that Sox4, which encodes a member of the Sry-related high-mobility group box protein family, was consistently upregulated in all three analysed models of neuropathy. Moreover, Sox4 showed a peak in its expression during development that corresponded with the onset of myelination. To gain further insights into the role of Sox4 in PNS development, we generated a transgenic mouse that specifically overexpresses Sox4 in Schwann cells. Sox4 overexpression led to a temporary delay in PNS myelination without affecting axonal sorting. Importantly, we observed that, whereas Sox4 mRNA could be efficiently overexpressed, Sox4 protein expression in Schwann cells was strictly regulated. Finally, our data showed that enforced expression of Sox4 in the mouse model for Charcot-Marie-Tooth 4C aggravated its neuropathic phenotype. Together, these observations reveal that Sox4 contributes to the regulation of Schwann cell myelination, and also indicates its involvement in the pathophysiology of peripheral neuropathies.

Keywords: Charcot-Marie-Tooth 4C; SRY-related high-mobility group box gene 4; peripheral neuropathy; transcription factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Animals, Newborn
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Carrier Proteins / genetics
  • Disease Models, Animal
  • Gene Expression Regulation, Developmental / genetics*
  • HEK293 Cells
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Mice
  • Mice, Transgenic
  • Mutation / genetics
  • Myelin Sheath / metabolism*
  • Peripheral Nervous System / metabolism*
  • Peripheral Nervous System Diseases / genetics
  • Peripheral Nervous System Diseases / metabolism
  • RNA, Messenger / genetics
  • SOXC Transcription Factors / genetics
  • SOXC Transcription Factors / metabolism*
  • Schwann Cells / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transfection

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Luminescent Proteins
  • RNA, Messenger
  • SOXC Transcription Factors
  • Sh3tc2 protein, mouse
  • Sox4 protein, mouse
  • Transcription Factors
  • yellow fluorescent protein, Bacteria