Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae

E Anne Hatmaker; Phillip A Wadl; Kristie Mantooth; Brian E Scheffler; Bonnie H Ownley; Robert N Trigiano

doi:10.3732/apps.1400123

Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae

Appl Plant Sci. 2015 Apr 7;3(4):apps.1400123. doi: 10.3732/apps.1400123. eCollection 2015 Apr.

Authors

E Anne Hatmaker¹, Phillip A Wadl¹, Kristie Mantooth¹, Brian E Scheffler², Bonnie H Ownley¹, Robert N Trigiano¹

Affiliations

¹ Department of Entomology and Plant Pathology, University of Tennessee, 2505 E. J. Chapman Drive, Knoxville, Tennessee 37996-4560 USA.
² USDA-ARS Genomics and Bioinformatics Research Unit, 141 Experiment Station Road, P.O. Box 36, Stoneville, Mississippi 38776-0038 USA.

Abstract

Premise of the study: We developed microsatellites from Fothergilla ×intermedia to establish loci capable of distinguishing species and cultivars, and to assess genetic diversity for use by ornamental breeders and to transfer within Hamamelidaceae.

Methods and results: We sequenced a small insert genomic library enriched for microsatellites to develop 12 polymorphic microsatellite loci. The number of alleles detected ranged from four to 15 across five genera within Hamamelidaceae. Shannon's information index ranged from 0.07 to 0.14.

Conclusions: These microsatellite loci provide a set of markers to evaluate genetic diversity of natural and cultivated collections and assist ornamental plant breeders for genetic studies of five popular genera of woody ornamental plants.

Keywords: Corylopsis; Hamamelidaceae; Hamamelis; Loropetalum; Parrotia; simple sequence repeats.