Objective: To investigate specific proteins in ultraviolet (UV)-induced acute damage of human fibroblasts using proteinchip with surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).
Methods: CM10 Proteinchip and SELDI-TOF-MS were used for detecting protein samples of non-irradiated group, UVA (2.5 J/cm(2))-irradiated group and UVB (100 mJ/cm(2))-irradiated group of normal human fibroblasts.
Results: Ten protein peaks were down-regulated and 8 protein peaks up-regulated in fibroblasts after UV irradiation. By searching in SWISS-PROT and TrEMBLE databases, the peaks of 11 320.1 and 8 574.3 accorded with those of caspase-7 precursor and cytochrome C oxidase polypeptide VIc precursor proteins.
Conclusion: As a quick and convenient high-throughout proteomic analytic method, SELDI-TOF-MS plus proteinchip offers a unique platform for detecting dermal photodamage.