The LPS-Pretreated MSCs Supply a Positive Microenvironment for Tumor Cell Proliferation and Clone Formation

Curr Protein Pept Sci. 2015;16(4):295-300. doi: 10.2174/138920371604150429152720.

Abstract

In this study, the culture supernatant of LPS-treated MSCs was collected and served as a conditioned medium (CM). It was found that the LPS-CM promoted the proliferation of tumor cells (SKBR3, MDA-MB-231, A549, 95D and HepG2). In addition, the colony formation ability was also enhanced by LPS-CM incubation. The inflammatory cytokines, IL-6 and IL-8, were demonstrated to be up-regulated in the LPS-CM, which we supposed to function in the tumor-growth promotion in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Humans
  • Interleukin-6 / metabolism
  • Interleukin-8 / metabolism
  • Lipopolysaccharides / pharmacology*
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / drug effects*
  • Mesenchymal Stem Cells / metabolism
  • NF-kappa B / metabolism
  • Signal Transduction / drug effects
  • Tumor Microenvironment / drug effects*
  • Up-Regulation / drug effects

Substances

  • Interleukin-6
  • Interleukin-8
  • Lipopolysaccharides
  • NF-kappa B