Inducing cell growth arrest and apoptosis by silencing long non-coding RNA PCAT-1 in human bladder cancer

Tumour Biol. 2015 Sep;36(10):7685-9. doi: 10.1007/s13277-015-3490-3. Epub 2015 May 2.

Abstract

Long non-coding RNAs (lncRNAs) are a class of non-coding RNAs that play important roles in cancer development and progression. Prostate cancer-associated transcript 1 (PCAT-1) is a novel lncRNA that promotes cell proliferation in prostate cancer. We hypothesized that PCAT-1 also have roles in bladder cancer. In this study, we found that PCAT-1 was up-regulated in bladder cancer compared to paired normal urothelium. Cell proliferation inhibition and apoptosis induction were also observed in PCAT-1 small hairpin RNA (shRNA)-transfected bladder cancer T24 and 5637 cells. Our data suggest that PCAT-1 plays oncogenic roles and can be used as a therapeutic target for treating human bladder cancer.

Keywords: Apoptosis; Bladder cancer; PCAT-1; Proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics*
  • Cell Line, Tumor
  • Cell Proliferation / genetics*
  • Gene Silencing / physiology*
  • Humans
  • RNA, Long Noncoding / genetics*
  • RNA, Small Interfering / genetics
  • Up-Regulation / genetics
  • Urinary Bladder Neoplasms / genetics*
  • Urothelium / pathology

Substances

  • PCAT-1 lncRNA, human
  • RNA, Long Noncoding
  • RNA, Small Interfering