MicroRNA-19a regulates proliferation and apoptosis of castration-resistant prostate cancer cells by targeting BTG1

FEBS Lett. 2015 Jun 4;589(13):1485-90. doi: 10.1016/j.febslet.2015.04.037. Epub 2015 Apr 30.

Abstract

MicroRNAs (miRNAs) play a significant role in tumor development. Recent studies indicate that miRNAs are implicated in prostate cancer (PCa). In this study, we found that miR-19a expression was significantly increased in castration-resistant prostate cancer (CRPC) tissues compared with androgen-dependent prostate cancer (ADPC) tissues. We found that inhibiting the overexpression of miR-19a in CRPC cells suppressed proliferation and increased apoptosis. Additionally, we found that miR-19a repressed BTG1 expression by binding to its 3'-untranslated region. The overexpression of BTG1 in CRPC cells significantly suppressed proliferation and increased apoptosis. We conclude that miR-19a regulates proliferation and apoptosis of CRPC cells by directly targeting the tumor suppressor gene BTG1.

Keywords: Apoptosis; BTG1; MiR-19a; Proliferation; Prostate cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Animals
  • Apoptosis / genetics*
  • Blotting, Western
  • Cell Line, Tumor
  • Cell Proliferation / genetics*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Mice, Inbred BALB C
  • Mice, Nude
  • MicroRNAs / genetics*
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / metabolism
  • Oligoribonucleotides, Antisense / genetics
  • Prostatic Neoplasms, Castration-Resistant / genetics*
  • Prostatic Neoplasms, Castration-Resistant / pathology
  • Prostatic Neoplasms, Castration-Resistant / therapy
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Burden / genetics
  • Xenograft Model Antitumor Assays

Substances

  • 3' Untranslated Regions
  • MIRN19A microRNA, human
  • MicroRNAs
  • Neoplasm Proteins
  • Oligoribonucleotides, Antisense
  • BTG1 protein, human