We describe novel tools, bioluminescent whole-cell reporter gene assays, for facilitating the use of natural products in antimicrobial drug discovery. As proof-of-concept, a plant extract library was screened and follow-up experiments were carried out. Primary results can be obtained in 2-4h with high sensitivity, leading to significant improvements of the process.
Keywords: Antibacterial; Bioluminescence; Cell-based assay; Escherichia coli; Natural products; Screening.
Copyright © 2015 Elsevier B.V. All rights reserved.