Characterisation of a multidrug-resistant Bacteroides fragilis isolate recovered from blood of a patient in Denmark using whole-genome sequencing

Int J Antimicrob Agents. 2015 Jul;46(1):117-20. doi: 10.1016/j.ijantimicag.2015.02.024. Epub 2015 Apr 17.

Abstract

Here we describe a patient undergoing extensive abdominal surgery and hyperthermic intraperitoneal chemotherapy due to primary adenocarcinoma in the sigmoid colon with peritoneal carcinomatosis. During hospitalisation the patient suffered from bacteraemia with a multidrug-resistant Bacteroides fragilis isolate. Whole-genome sequencing of the isolate resulted in identification of nimE, cfiA and ermF genes corresponding to metronidazole, carbapenem and clindamycin resistance.

Keywords: Bacteroides fragilis; Multidrug resistance; cfiA; ermF; nimE; tetQ.

Publication types

  • Case Reports

MeSH terms

  • Bacteremia / microbiology*
  • Bacteroides Infections / microbiology*
  • Bacteroides fragilis / classification
  • Bacteroides fragilis / drug effects*
  • Bacteroides fragilis / genetics
  • Bacteroides fragilis / isolation & purification
  • Blood / microbiology*
  • Colonic Neoplasms / complications
  • Denmark
  • Drug Resistance, Multiple, Bacterial*
  • Female
  • Genes, Bacterial
  • Genome, Bacterial*
  • Humans
  • Middle Aged
  • Molecular Sequence Data
  • Peritoneal Neoplasms / complications
  • Peritoneal Neoplasms / secondary
  • Sequence Analysis, DNA*

Associated data

  • GENBANK/JMZX01000000