Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

J Clin Microbiol. 2015 Jul;53(7):2355-8. doi: 10.1128/JCM.00494-15. Epub 2015 May 6.

Abstract

We determined that the Vitek MS Plus matrix-assisted laser desorption ionization-time of flight mass spectrometry using research-use-only (RUO) v.4.12 and in vitro-diagnostic (IVD) v.3.0 databases accurately identified 41 Mycobacterium abscessus subsp. abscessus and 13 M. abscessus subsp. massiliense isolates identified by whole-genome sequencing to the species but not the subspecies level, from Middlebrook 7H11 and Burkholderia cepacia selective agars. Peak analysis revealed three peaks potentially able to differentiate between subspecies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Microbiological Techniques / methods*
  • Mycobacterium Infections, Nontuberculous / diagnosis
  • Mycobacterium Infections, Nontuberculous / microbiology
  • Nontuberculous Mycobacteria / chemistry*
  • Nontuberculous Mycobacteria / classification*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*