Value of caffeic acid phenethyl ester pretreatment in experimental sepsis model in rats

Mediators Inflamm. 2015:2015:810948. doi: 10.1155/2015/810948. Epub 2015 Apr 8.

Abstract

Background and aim: The aim of this study was to determine the actions of caffeic acid phenethyl ester (CAPE) on the changes of endothelin-1 (ET-1) level, tumor necrosis factor- (TNF-) alpha, and oxidative stress parameters such as superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels in experimental sepsis model in rats.

Materials and methods: Twenty-four rats were randomly divided into three experimental groups: sham (group 1), sepsis (group 2), and sepsis + CAPE (group 3), n = 8 each. CAPE was administered (10 µmol/kg) intraperitoneally to group 3 before sepsis induction. Serum ET-1, serum TNF-alpha, tissue SOD activity, and tissue MDA levels were measured in all groups.

Results: Pretreatment with CAPE decreased ET-1, TNF-alpha, and MDA levels in sepsis induced rats. Additionally SOD activities were higher in rats pretreated with CAPE after sepsis induction.

Conclusion: Our results demonstrate that CAPE may have a beneficial effect on ET and TNF-alpha levels and oxidative stress parameters induced by sepsis in experimental rat models. Therefore treatment with CAPE can be used to avoid devastating effects of sepsis.

MeSH terms

  • Animals
  • Caffeic Acids / therapeutic use*
  • Disease Models, Animal
  • Endothelin-1 / blood
  • Inflammation / metabolism
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress
  • Oxygen / chemistry
  • Phenylethyl Alcohol / analogs & derivatives*
  • Phenylethyl Alcohol / therapeutic use
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Sepsis / drug therapy*
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Caffeic Acids
  • Endothelin-1
  • Thiobarbituric Acid Reactive Substances
  • Tumor Necrosis Factor-alpha
  • Malondialdehyde
  • Superoxide Dismutase
  • caffeic acid phenethyl ester
  • Phenylethyl Alcohol
  • Oxygen