Full genomic characterization and phylogenetic analysis of a zoonotic human G8P[14] rotavirus strain detected in a sample from Guatemala

Infect Genet Evol. 2015 Jul:33:206-11. doi: 10.1016/j.meegid.2015.05.004. Epub 2015 May 5.

Abstract

We report the genomic characterization of a rare human G8P[14] rotavirus strain, identified in a stool sample from Guatemala (GTM) during routine rotavirus surveillance. This strain was designated as RVA/Human-wt/GTM/2009726790/2009/G8P[14], with a genomic constellation of G8-P[14]-I2-R2-C2-M2-A13-N2-T6-E2-H3. The VP4 gene occupied lineage VII within the P[14] genotype. Phylogenetic analysis of each genome segment revealed close relatedness to several zoonotic simian, guanaco and bovine strains. Our findings suggest that strain RVA/Human-wt/GTM/2009726790/2009/G8P[14] is an example of a direct zoonotic transmission event. The results of this study reinforce the potential role of interspecies transmission and reassortment in generating novel and rare rotavirus strains which infect humans.

Keywords: G8P[14]; Interspecies transmission; Reassortant; Rotavirus.

MeSH terms

  • Animals
  • Genes, Viral
  • Genome, Viral*
  • Genomics*
  • Guatemala / epidemiology
  • Humans
  • Open Reading Frames
  • Phylogeny*
  • Rotavirus / classification*
  • Rotavirus / genetics*
  • Rotavirus Infections / epidemiology*
  • Rotavirus Infections / transmission
  • Rotavirus Infections / virology*
  • Zoonoses / virology