Regulation of OsmiR156h through Alternative Polyadenylation Improves Grain Yield in Rice

PLoS One. 2015 May 8;10(5):e0126154. doi: 10.1371/journal.pone.0126154. eCollection 2015.

Abstract

Substantial increases in grain yield of cereal crops are required to feed a growing human population. Here we show that a natural variant of SEMIDWARF AND HIGH-TILLERING (SDT) increases harvest index and grain productivity in rice. Gain-of-function sdt mutation has a shortened polyadenylation tail on the OsmiR156h microRNA precursor, which cause the up-regulation of OsmiR156h. The plants carrying the semidominant sdt allele exhibit reduced plant height, enhanced lodging resistance, increased tiller numbers per plant, and resulting in an increased grain yield. We also show that combining the sdt allele with the OsSPL14WFP allele can be effective in simultaneously improving tillering capacity and panicle branching, thereby leading to higher harvest index and grain yield. Most importantly, pyramiding of the sdt allele and the green revolution gene sd1 enhances grain yield by about 20% in hybrid rice breeding. Our results suggest that the manipulation of the polyadenylation status of OsmiR156 represents a novel strategy for improving the yield potential of rice over what is currently achievable.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Base Sequence
  • Cloning, Molecular
  • Edible Grain / growth & development
  • MicroRNAs / chemistry
  • MicroRNAs / metabolism*
  • Oryza / genetics*
  • Oryza / growth & development
  • Oryza / metabolism
  • Phenotype
  • Plant Breeding
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / growth & development
  • Plants, Genetically Modified / metabolism
  • Polyadenylation
  • Sequence Alignment
  • Up-Regulation

Substances

  • MicroRNAs
  • Plant Proteins

Grants and funding

This work was supported by grants from National Natural Science Foundation of China (31130070, 91335207 and 31301297), the Ministry of Science and Technology of China (2012AA10A301) and Chinese Academy of Sciences (KSCX2-EW-N-01, KJCX2-EW-N05 and KJCX2-YW-N34).