Identification of T cell clones without the need for sequencing

J Immunol Methods. 2015 Sep:424:28-31. doi: 10.1016/j.jim.2015.04.018. Epub 2015 May 8.

Abstract

The brainbow recombination fluorescent protein system has been used for a multitude of applications in fate and lineage tracking. Here, we use a mouse with a ubiquitously expressed brainbow construct, termed the Confetti mouse, to perform T lymphocyte cell lineage tracking. We demonstrate that antigen-specific T lymphocyte clonotypes can be identified and phenotyped using flow cytometry instead of performing expensive and time-consuming methods of single cell sequencing.

Keywords: Clonotype; Lineage tracking; T cell receptor.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Clone Cells
  • Flow Cytometry / methods
  • Gene Expression
  • Genes, Reporter
  • Mice
  • Mice, Transgenic
  • Receptors, Antigen, T-Cell / genetics
  • Sequence Analysis, DNA
  • Single-Cell Analysis / methods*
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism*
  • Tamoxifen / administration & dosage
  • Tamoxifen / pharmacology

Substances

  • Receptors, Antigen, T-Cell
  • Tamoxifen