Assessment of four protocols for rapid bacterial identification from positive blood culture pellets by matrix-assisted laser desorption ionization-time of flight mass spectrometry (Vitek® MS)

J Microbiol Methods. 2015 Aug:115:54-6. doi: 10.1016/j.mimet.2015.05.010. Epub 2015 May 9.

Abstract

In this study, we developed and compared four protocols to prepare a bacterial pellet from 944 positive blood cultures for direct MALDI-TOF mass spectrometry Vitek® MS analysis. Protocol 4, tested on 200 monomicrobial samples, allowed 83% of bacterial identification. This easy, fast, cheap and accurate method is promising in daily practice, especially to limit broad range antibiotic treatment.

Keywords: Blood bottle culture; MALDI-TOF; Rapid identification.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteria / chemistry
  • Bacteria / classification
  • Bacteria / isolation & purification*
  • Bacterial Typing Techniques / economics
  • Bacterial Typing Techniques / methods*
  • Blood / microbiology*
  • Humans
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / economics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Tandem Mass Spectrometry / economics
  • Tandem Mass Spectrometry / methods*