IL-15-secreting γδT cells induce memory T cells in experimental allergic encephalomyelitis (EAE) mice

Xiaoqian Wang; Yinxiang Wei; Xiaoling Liu; Chen Xing; Gencheng Han; Guojiang Chen; Chunmei Hou; Ivy M Dambuza; Beifen Shen; Yan Li; He Xiao; Renxi Wang

doi:10.1016/j.molimm.2015.04.021

IL-15-secreting γδT cells induce memory T cells in experimental allergic encephalomyelitis (EAE) mice

Mol Immunol. 2015 Aug;66(2):402-8. doi: 10.1016/j.molimm.2015.04.021. Epub 2015 May 18.

Authors

Xiaoqian Wang¹, Yinxiang Wei², Xiaoling Liu³, Chen Xing¹, Gencheng Han¹, Guojiang Chen¹, Chunmei Hou¹, Ivy M Dambuza⁴, Beifen Shen¹, Yan Li¹, He Xiao⁵, Renxi Wang⁶

Affiliations

¹ Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China.
² Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China; Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310013, China.
³ Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China; Department of Mephrology, The 307th Hospital of Chinese People's Liberation Army, Beijing 100850, China.
⁴ Molecular Immunology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892-1857, USA.
⁵ Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China. Electronic address: [email protected].
⁶ Laboratory of Immunology, Institute of Basic Medical Sciences, PO Box 130 (3), Taiping Road #27, Beijing 100850, China. Electronic address: [email protected].

PMID: 25974878
DOI: 10.1016/j.molimm.2015.04.021

Abstract

With the most recent data suggesting γδT cells as primary producers of the pro-inflammatory autoimmune-associated cytokine, the relationship between γδT cells and Th17 in experimental allergic encephalitis (EAE) mice requires more extensive investigation. By flow cytometry and qPCR, we identified a new subset of IL-15-secreting γδT (γδT15) cells that increased in EAE mice. The capacity of IL-15-secreting γδT cells inducing memory T cells and memory T cells inducing IL-17(+)Th17 was examined by transferring into EAE mice and 7-week-old female nude mice, respectively. We found that γδT15 induced CD44(hi) memory T cells by secreting IL-15. γδT15-induced memory T cells induced EAE by transforming into pathogenic Th17 cells. The data suggest that a new subset of IL-15-secreting γδT cells mediated the production of memory T cells which transformed into pathogenic Th17 cells in EAE mice.

Keywords: EAE; IL-15; Multiple sclerosis; Th17; γδT Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Encephalomyelitis, Autoimmune, Experimental / chemically induced
Encephalomyelitis, Autoimmune, Experimental / genetics
Encephalomyelitis, Autoimmune, Experimental / immunology*
Encephalomyelitis, Autoimmune, Experimental / pathology
Female
Gene Expression
Hyaluronan Receptors / genetics
Hyaluronan Receptors / immunology
Immunologic Memory / genetics*
Immunophenotyping
Interleukin-15 / genetics*
Interleukin-15 / immunology
Interleukin-17 / genetics
Interleukin-17 / immunology
Mice
Mice, Inbred C57BL
Mice, Nude
Myelin-Oligodendrocyte Glycoprotein
Peptide Fragments
Receptors, Antigen, T-Cell, gamma-delta / genetics*
Receptors, Antigen, T-Cell, gamma-delta / immunology
Th1 Cells / immunology
Th1 Cells / pathology
Th17 Cells / immunology*
Th17 Cells / pathology

Substances

Cd44 protein, mouse
Hyaluronan Receptors
Interleukin-15
Interleukin-17
Myelin-Oligodendrocyte Glycoprotein
Peptide Fragments
Receptors, Antigen, T-Cell, gamma-delta
myelin oligodendrocyte glycoprotein (35-55)